DNA: PCR and Electrophoresis

This laboratory procedure is known as

• CLONING

• gel electrophoresis

• chromatography

• use of a dichotomous key

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

During gel electrophoresis, DNA moves from the ___ end to the ___ end.

• positive, negative
• negative, positive

Tags

NGSS.HS-PS2-5

What applications can gel electrophoresis be used for?

• Parental Testing
• Criminal Investigations
• Sequence Endangered Species DNA
• All of the above

Tags

NGSS.HS-LS1-1

IN PCR why is the DNA heated?

• To split the DNA strand

• To remove the DNA strand

• To correct the DNA strand

At what temperature does the Anneal step of PCR occur?

• 94-96° C
• 50-65° C
• 72° C
• Any temperature

What happens during the Anneal step of PCR?

• Primers are created.
• The primers attach to the target DNA region.
• Primers copy the new DNA strand.
• Primers sequence DNA

What type of bonds hold the two strands of DNA together?

• Hydrogen bonds
• Covalent bonds
• Dipole-Dipole bonds
• Ionic bonds