MI 4.1.4 Quiz Review (Quizizz)

The purpose of SDS in protein electrophoresis is to

• invert (flip) the hydrophobic regions to the outside of the protein.

• catabolize (break down) protein into individual amino acids.

• coat the protein with negative charges.

• wash the protein.

• denature (unfold) the protein.

The gel generally used in protein electrophoresis is

• polyacrylamide.

• 2.0% agarose.

• gelatin.

• agar.

• 0.8% agarose.

Protein electrophoresis is different than nucleotide (DNA) electrophoresis in that in protein electrophoresis,

• proteins are, by default, negatively charged.

• proteins are usually a lot smaller.

• proteins are usually linearized, not folded.

• the units measured are in kDa.

• current flows from positive to negative.

A sample containing proteins that have not been gone through column chromatography most likely will result in a gel that contains

• two bands indicating GFP and beta-lactamase.

• multiple bands indicating different types of proteins present.

• a single band indicating pure GFP.

• a single band indicating pure bacterial proteins.

• multiple bands indicating fragmented or fractured GFP.

SDS stands for

• sodium dimethyl sulfonate.

• solvent dessicant solution.

• soapy dishwater solution.

• sodium dodecahedron salicylate.

• sodium dodecyl sulfate.

The molecular weight of GFP is

• 26.9 kb.

• 26.9 kDa.

• 26.9 Da.

• 29.6 kDa.

• not known.

Which substance is used to separate proteins by size?

• sodium docecyl sulfate

• calcium chloride

• 2-mercaptoethanol

• polyacrylamide

• wash buffer

2-mercaptoethanol is used primarily to

• give proteins a negative charge.

• convert the native form of proteins to the denatured form.

• separate proteins by size.

• break down proteins into amino acids.

• purify proteins.

Protein electrophoresis is different from DNA electrophoresis in that ____________ in protein electrophoresis.

• polyacrylamide is used

• agarose is used

• a vertical apparatus chamber is used

• a horizontal apparatus chamber is used

• the electric current flow is from positive to negative

To visualize proteins on a gel, it is best to

• use SybrSafe.

• refrigerate the gel overnight.

• use a protein stain and agitate the gel for a few hours.

• use a blue light box.

• use a protein stain and view immediately.