Recombinant DNA Technology

A cell is made up almost entirely of _______________. 

• carbohydrates
• proteins
• fats
• nucleic acids

Once the gene and plasmid have been cut with a restriction enzyme, another enzyme called _________ is used to join the pieces of DNA together.

• polymerase
• ligase
• dehydrogenase
• lactase

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• rDNA technology
• transferrence
• transformation
• rDNA plasmid

Which of the following is a protein that acts as DNA scissors?

• plasmid
• restriction enzyme
• ligase
• protein expression

Which of the following is an enzymatic protein that acts as DNA glue?

• plasmid
• restriction enzyme
• ligase
• protein expression

What is the most logical sequence of steps for splicing foreign DNA into a plasmid and inserting the plasmid into bacterium?
I) Transform bacteria with recombinant DNA molecule.
II) Cut the plasmid DNA using restriction enzymes.
III) Extract plasmid DNA from bacterial cells.
IV) Hydrogen-bond the plasmid DNA to non-plasmid DNA fragments.
V) Use ligase to seal plasmid DNA to non-plasmid DNA

• I, II, IV, III, V
• III, II, IV, V, I
• II, III, V, IV, I
• III, IV, V, I, II

The unpaired nucleotides produced by the action of restriction enzymes are referred to as

• sticky end.
• base sequence.
• single strands.
• restriction fragments.