Chapter 10 Genetics

Which gene is incorporated into plasmids to detect recombinant cells?

• restriction endonuclease

• virus receptors

• a gene for antibiotic resistance

• reverse transcriptase

Which of the following is not essential to carry out the polymerase chain reaction?

• primers
• DNA polymerase
• gel electrophoresis
• high temperature

The function of the ligase is to

• rejoin segments of DNA
• make longitudinal cuts in DNA
• synthesize cDNA
• break down ligaments

The pathogen of plant roots that is used as cleaning host is

• Pseudomonas
• Agrobacterium
• Escherichia coli
• Saccharomyces cerevisiae

Which of the following is or are required for cloning a gene?

• restriction endonuclease
• vector
• cloning host
• all of these

For which of the following would a nucleic acid probe not be used?

• locating a gene on a chromosome
• developing a Southern blot
• identifying a microorganism
• constructing a recombinant plasmid

reverse transcriptase

• enzyme that transcribes RNA into DNA
• no
• no
• no

Taq polymerase

• thermostable enzyme for synthesizing DNA
• no
• no
• no

primer

• oligonucleotide that initiates the PCR
• no
• no
• no

restriction endonuclease

• enzyme that snips DNA at palindromes
• no
• no
• no