Waters HPLC Troubleshooting and Quality Parts Seminar

Which of the following are the benefits of Waters Quality Parts?

• Waters Quality Parts are tested on Waters system – designed specifically for LC optimal performance

• Critical clean process during manufacturing to ensure parts are handled properly – greatly reduces chances of contamination

• Peace of mind that the valuable LC investment is protected – system warranty is not invalidated by installing third party parts

• All of the above

Which statement is correct?

• Waters recommends a Performance Maintenance once every two years

• All the critical wear-and-tear components are replaced during the Performance Maintenance regardless of the parts’ conditions

• User self-performed maintenance is sufficient to ensure system performance

• Quality of the mobile phase will not affect the quality of chromatogram

Which is not correct about the troubleshooting workflow?

• Having a system benchmark helps me to have a good chromatogram to refer to when there is a suspected LC problem

• I am encouraged to use my own sample to setup as system benchmark

• Failure fingerprint should have information on how the chromatogram fails and what causes it to fail

• Comparing between failure fingerprint helps me to remove any inconsistent failure causes

What is/are the cause(s) of peak shape problem?

• Poor fitting

• Column void

• Plugged column

• Silanol activity from column

• All of the Above

QCRM from Waters can be used to? (Choose all the answers that applies)

• Benchmark system performance

• Perform routine PQ checks

• Diagnose/troubleshoot system issues

• Tell which parts to change of the system

Under the current USP 621 guideline, which is the chromatographic parameter that can be adjusted without revalidation?

• Gradient method

• Detector wavelength

• Different L listing column

• Column length and particle size for isocratic method

Part-part manakah yang kemungkinan besar bermasalah Jika RSD RT tidak masuk (>2%) Pada system HPLC anda?

• Solvent Filter, Inlet Filter, Checkvalve dan Seal-seal Plunger

• Solvent filter, Needle, needle Seal dan sample Syringe

• Inlet Filter, Check Valve, Injector valve dan flowcell detector

• Needle Seal, needle, Sample Syringe dan Injector valve

Part-part manakah yang kemungkinan besar bermasalah Jika RSD Area tidak masuk (>2%) Pada system HPLC anda?

• Solvent Filter, Inlet Filter, Checkvalve dan Seal-seal Plunger

• Solvent filter, Needle, needle Seal dan sample Syringe

• Inlet Filter, Check Valve, Injector valve dan flowcell detector

• Needle Seals, needle and Sample Syringe

Hal-hal berikut ini dapat menyebabkan peak yang dihasilkan mempunyai nilai tailing yang tinggi, Kecuali?

• Interaksi sampel dengan stationary phase dalam kolom

• Pemasangan tubing pada kolom tidak sempurna

• Kolom terdegradasi/rusak

• Sampel menguap

Manakah penyebab yang paling sering kolom mengalami blocking/ mampet?

• Algae / Bakteri

• Kesalahan memilih filter

• Fasa gerak buffer

• Semua jawaban benar