SB015 RECOMBINANT DNA TECHNOLOGY SESI 2022/2023

An enzyme that cut DNA molecules at a specific restriction site is called

• DNA polymerase

• RNA polymerase

• Restriction enzyme

• tRNA

Which of the following sequences in double stranded DNA is most likely to produce sticky end fragments when treated with a restriction enzyme

• 5'-CTAAGC-3'

  3'-GATTCG-5'

• 5'-GAATTC-3'

  3'-CTTAAG-5'

• 5'-TTCCTT-3'

  3-AAGGAA-5'

• 5'-TCAGTC-3'

  3'-AGTCAG-5'

In order to obtain the gene of interest from the DNA of organism

• The DNA must be cut only at one site near the gene of interest.

• The DNA must be cut at two sites to get a DNA fragment that contains from the gene of interest.

• The DNA must be cut at any two sites to get any DNA fragments from the organism

• The DNA must not be cut at all, so that the gene of interest is retaine

What is a cloning vector?

• An agent that transfers a pathogen

• An agent that transfers a foreign gene from one DNA molecule to another.

• An agent that transfers a DNA segment to a RNA molecule

• An agent that transfers a RNA segment to a DNA molecule

Plasmid are important in biotechnology because they are

• a vehicle for the insertion of foreign DNA into bacteria

• surfaces for respiratory process in bacteria

• recognition site on recombinant DNA strand

• enzyme cuts DNA into restriction fragments

The method used to produce single strand of DNA

• is known as amplification

• is heating a solution of DNA

• uses restriction enzymes

• isolate DNA molecules from the nucleus

Which of the following statements describes a modifying enzyme?

• It cuts the DNA to obtain the gene of interest

• It breaks the DNA molecule to produce multiple fragments

• It stimulates the replication of a recombinant DNA.

• It seals the inserted gene of interest to an open plasmid

The figure below shows steps in manipulating bacterial cells to produce insulin. What

are enzyme X, process Y and Z?

• X : Restriction enzyme

  Y : Transformation

  Z : Screening

• X : DNA ligase

  Y : Screening

  Z : Amplification

• X : DNA ligase

  Y : Transformation

  Z : Screening

• X : Restriction enzyme

  Y : Insertion

  Z : Screening

Which of the following is TRUE regarding screening by using X-gal? (Your can tick more than one answer)

• Antibiotics will prevent the growth of bacteria without plasmid.

• Bacteria cells with recombinant plasmids are resistant to antibiotics and unable to hydrolyse X-gal

• Bacteria cells which contain non-recombinant plasmids are resistant to antibiotics and unable to hydrolyse X-gal.

The following steps involved in gene cloning producers. Rearrange them into the correct sequences

 

I.    Transformation and amplification

II.   Screen for bacteria that contain recombinant DNA.

III.   Insert DNA fragment into the cut bacterial plasmid.

IV.  Cleave the plasmid and human DNA with the same restriction enzyme.

V.  Isolate the bacterial plasmid and human DNA.

• I, II, III, IV, V

• V, IV, III, I, II 

• V, IV, II, III, I

• IV, V, III, I, II