Lateral Flow Assay Development

What is the core principle of protein purification by AKTA system?

• Liquid chromatography

• Solvent-solvent extraction

• Mass spectrophotometry

• Recombinant technology

Which of the following is an application example of protein purification?

• Extraction of total protein from cell lysate

• Gene expression studies

• Antigen-receptor interaction studies

• Isolation of antibody from serum or plasma

Which of the following technique is not suitable as first step in protein purification?

• Size exclusion chromatography

• Hydrophobic interaction

• Affinity chromatography

• Ion-exchange chromatography

Which of the following is the ultimate advantage of using prepacked column on an automated system in protein purification?

• Better resolution of separation

• Higher protein purity

• Higher protein yield

• Better reproducibility

What can we use to analyze the purity of fraction after purification?

• SDS-PAGE gel with Coomassie stain

• Western blot

• PCR

• Ultrafiltration

What is the first component in lateral flow kit:

• Glass/Cellulose Fiber Sample Pad

• Glass Fiber Conjugate Pad

• Nitrocellulose Membrane

• Cellulose Fiber Wicking Pad

What is the key considerations select right sample pad, conjugate pad and absorbent pad

• Thickness

• Wicking Rate

• Water absorption

• All of the above

What is the key considerations select right Nitrocellulose membrane?

• Capillary rise time

• Total caliper (of membrane)

• Membrane backing

• All of the above

What is the most critical component in a lateral flow assembly

• Glass/Cellulose Fiber Sample Pad

• Glass Fiber Conjugate Pad

• Nitrocellulose Membrane

• Cellulose Fiber Wicking Pad

Does Cytiva offer lateral Stater Pack?

• Yes

• No