Chromatography Review

What is chromatography?

• Prepping a plasmid for transformation

• Separating components of a mixture according to the molecules' properties

• Analyzing a mixture through spectrophotometry

• Creating more copies of a DNA sequence

What type of chromatography is depicted by the illustration?

• Gel Filtration Chromatography

• Column Chromatography

• HPLC

• Thin Layer Chromatography

Which of the following would give greater resolution in Gel Chromatography?

• increased temperature

• longer column

• fast flow rate

• increased pressure

The mobile phase in paper chromatography is typically a

• plasma
• solid
• gas
• liquid

The retention factor is used

• to zero the chromatography scale
• to identify unknown compounds in a sample
• to retain solvent
• as a starting tool for chromatography

The medium used for chromatography is called the...

Which of the following affects the resolving power of a column chromatography apparatus?

• Type and concentration of resin and buffer

• Concentration of sample mixture

• Length of column

• Flow rate and pressure

In Size Exclusion Chromatography, the large molecules will exit the column...

• FIRST

• LAST

Which type of chromatography is dependent on antibody-antigen interactions?

• Size Exclusion

• Ion- Exchange

• Gas Chromatography

• Affinity

In Thin Layer Chromatography, how is the Rf value calculated?