Activity 1.1.6 Review

The process of ______ is used to create more copies of a specific sequence of DNA.

• PCR

• Gel Electrophoresis

• Micropipetting

• Digestion

Restriction enzymes come from ...

• viruses

• bacteria

• plants

• animals

The job of restriction enzymes is to ...

• copy the DNA.

• cut the DNA at specific locations.

• remove the DNA.

• organize the DNA.

If a restriction enzyme cuts the DNA 3 times, how many recognition sites are there on this DNA?

• 1

• 2

• 3

• 4

If a restriction enzyme cuts the DNA 3 times, how many RFLPs were made?

• 1

• 2

• 3

• 4

If a restriction enzyme makes a cut that is straight up and down through the double strand of DNA, this is called creating ______ ends.

• sticky

• blunt

• straight

• organized

The process that separates the cut fragments of DNA by their size and compares them is called ____.

• PCR

• digestion

• gel electrophoresis

• restriction enzymes

DNA has a _____ charge.

• positive

• negative

• neutral

• unknown

The area on the gel where you load the DNA is called the ____.

• wells

• positive pole

• digestion site

• loading site

The larger fragments of DNA will ...

• remain closer to the wells.

• travel towards the + pole of the gel.

• not move at all.

• travel farther than the smaller fragments.