Enzyme Factors

In experiment 1, Enzyme A and a large amount of its substrate are exposed to temperatures ranging from 0 degrees Celsius to 100 degrees Celsius. In experiment 2, Enzyme A and a large amount of its substrate are exposed to varying pH values from 1 to 14.

During experiment 1, the highest rate of reaction will most likely be observed at

• 0 degrees celsius.

• A moderately warm temperature.

• 100 degrees celsius

• Not enough information

Answer explanation

Most human enzymes work fastest at body temperature (approximately 37 deg C) but there are some bacteria that have enzymes that work fastest in MUCH warmer temperatures without denaturing.

In experiment 1, Enzyme A and a large amount of its substrate are exposed to temperatures ranging from 0 degrees Celsius to 100 degrees Celsius. In experiment 2, Enzyme A and a large amount of its substrate are exposed to varying pH values from 1 to 14.

During experiment 1, the enzyme will most likely become denatured and stop catalyzing the reaction at

• 0 degrees Celsius

• A moderate temperature

• 100 degrees Celsius

• Not enough information

In experiment 1, Enzyme A and a large amount of its substrate are exposed to temperatures ranging from 0 degrees Celsius to 100 degrees Celsius. In experiment 2, Enzyme A and a large amount of its substrate are exposed to varying pH values from 1 to 14.

During experiment 1, the rate of reaction was observed to increase as the temperatures increased from 15 degrees to 38 degrees. What explains this trend?

• more substrate molecules being produced

• The enzyme is converting thermal energy into chemical energy

• the enzyme is being denatured

• faster moving enzymes and substrates collide more often

In experiment 1, Enzyme A and a large amount of its substrate are exposed to temperatures ranging from 0 degrees Celsius to 100 degrees Celsius. In experiment 2, Enzyme A and a large amount of its substrate are exposed to varying pH values from 1 to 14.

During experiment 2, investigators observe that the enzyme shows no activity at certain pH values. Which pH value would be most likely to show no activity?

• 2

• 5

• 6

• 8

In experiment 1, Enzyme A and a large amount of its substrate are exposed to temperatures ranging from 0 degrees Celsius to 100 degrees Celsius. In experiment 2, Enzyme A and a large amount of its substrate are exposed to varying pH values from 1 to 14.

AFTER experiment 2, more substrate is added to Enzyme A in the pH 14 environment. The reaction rate will most likely

• remain high

• remain low

• increase, then remain constant

• increase, then decrease

Answer explanation

Enzymes in extreme pH are denatured. This is a permanent change to the shape and will prevent it from binding with the substrate.

In experiment 1, Enzyme A and a large amount of its substrate are exposed to temperatures ranging from 0 degrees Celsius to 100 degrees Celsius. In experiment 2, Enzyme A and a large amount of its substrate are exposed to varying pH values from 1 to 14.

During experiment 2, rate of reaction will ____________ as the pH values increase.

• Increase

• Decrease

• Increase then decrease

• Decrease then increase

Increasing substrate concentration will increase the rate of reaction because

• they substrates have more energy

• the substrates have less energy

• the enzymes become denatured

• more substrates will collide with the enzymes

Catalase is an enzyme that catalyzes the breakdown of hydrogen peroxide (H2O2) into water and oxygen. Does increasing the hydrogen peroxide concentration increase the rate of the reaction?

• Yes

• No

• Yes, until the enzymes become saturated

• Yes, until the enzymes runs out of energy

Scientists are looking to slow down the rate of a reaction so that will generate less heat. They can reduce the substrate concentration and they can reduce the enzyme concentration.

Decreasing the substrate concentration is most like

• decreasing the temperature in a factory

• decreasing the number of workers in a factory

• decreasing the working hours in a factory

• decreasing the raw materials available in a factory

Scientists are looking to slow down the rate of a reaction so that will generate less heat. They can reduce the substrate concentration and they can reduce the enzyme concentration.

Decreasing the enzyme concentration is most like

• decreasing the temperature in a factory

• decreasing the number of workers in a factory

• decreasing the number of hours in a factory

• decreasing the amount of raw materials in a factory