COVID-19 Diagnostics Quiz

Which of the following is FALSE about RAT extraction buffers?

• They maintain pH as CO2 accumulates in solution

• The basic detergent ruptures the cell in membrane

• They are optimized to isolate SARS-CoV-2 antigens

• The isolated nucleocapsid protein is methylated by buffer

• A HEPES-NaOH buffer is an example of a buffer system

Which of the following statements is TRUE about the LFA serological test?

• Only trained laboratory staff can perform this test

• A faded line means the tests are invalid

• At the conjugation pad, any SARS-CoV-2 antibodies in the sample bind to the gold-tagged SARS-CoV-2 antigens

• A positive test for IgM and negative for IgG indicates that the individual must have had the infection several weeks ago

• The LFA serological test provides both quantitative and qualitative analysis

What are the two pathways by which VOCs are produced during COVID-19 infection?

• 1. Cellular respiration of the host cell

  2. B-cell VOC emission as byproducts of antigen presentation

• 1. B-cell VOC emission as byproducts of antigen presentation

  2. Oxidative stress caused by host cell damage

• 1. VOC preconcentration by GCMS

  2. Cellular respiration of the host cell

• 1. Oxidative stress caused by host cell damage

  2. VOC preconcentration by GCMS

• SARS-CoV-2 does not influence VOC production

Which of the following about RT-PCR reactions are NOT TRUE?

• Reverse transcription is required before PCR reaction

• PCR has a 67% false-positive rating in the first five days

• In Taqman assays for fluorescence, probes contain a dye label and minor groove binders on the 5' end, and the quencher on the 3' end

• In Taqman assays for fluorescence, probes contain a dye label on the 5' end, and the quencher and minor groove binders on the 3' end

• Viral RNA needs to be extracted prior to PCR analysis