IB DNA Replication

Which enzyme relieves the strain on strands of DNA when they are separated?

• DNA polymerase III

• DNA Gyrase

• Helicase

• DNA polymerase I

State the enzyme that uncoils DNA by breaking the hydrogen bonds between complementary bases

Which enzyme seals the nick between fragments of DNA by forming a sugar-phosphate bond

• DNA polymerase III

• DNA primase

• DNA polymerase I

• DNA ligase

DNA replication takes place in which direction?

• 3' -> 5'

• 5' -> 5'

• 3' -> 3'

• 5' -> 3'

RNA primers are added to which strand?

Which enzyme replaces the RNA primer with a short section of DNA?

• DNA polymerase I

• DNA ligase

• Helicase

• Taq polymerase

Taq polymerase is used in PCR for which of these reasons?

• It breaks hydrogen bonds between bases

• It catalyses reactions quickly

• It does NOT denature at high temperatures

• It is cheap

Which sequence of bases would be complementary to this sequence on a primer?

AACCGUAGU

Fill in the missing word: DNA replication is ---- conservative beacuse an original strand is conserved in each of two new molecules of DNA

Which lab process is used to amplify minute quantities of DNA so that it can be analysed further?