Review ELISA, DNA sequencing

4.) During the ELISA test, what happens when the enzyme becomes attached to the substrate?

• a.) bind to primary antibody

• b.) destroy the antigen

• c.) color change

• d.) no antigens present

5.) In what order should you add the reagents to perform an ELISA test?

• a.) Primary antibody, antigen sample, enzyme-linked secondary antibody, substrate

• b.) Antigen sample, primary antibody, enzyme-linked secondary antibody, substrate

• c.) Substrate, enzyme-linked secondary antibody, antigen samples

• d.) Primary antibody, enzyme-linked secondary antibody, antigen samples, substrate

6.) ________ have surface _________ where _________ attach.

• a.) antibodies, pathogens, antigens

• b.) antigens, antibodies, pathogens

• c.) pathogens, antibodies, antigens

• d.) pathogens, antigens, antibodies

7.) An outbreak of a disease has been spreading through a small community. Using samples taken from infected patients, which of the following tests BEST enable doctors to identify an unknown pathogen from the outbreak?

• a.) DNA electrophoresis to compare samples

• b.) Sequence via Recombinant DNA methods

• c.) Automated sequencer to determine the DNA sequence, then compare to known organisms using BLAST.

• d.) Use PCR to amplify the genome of the infected patients, then give to test subjects.

9.) What career combines molecular biology and computer analysis?

• a.) diagnostic imaging

• b.) genetics

• c.) bioinformatics

• d.) microbiology

10.) Place the following processes for using DNA for bacterial identification in order:

1. obtain bacterial DNA from patient samples

2. Sequence the bacterial DNA by reading the order of fluorescent nucleotides

3. Use BLAST to match the bacterial sequence to known organisms

4. Use PCR to amplify (make more copies of) the bacterial DNA.

5. Use fluorescently tagged nucleotides for sequencing

• a.) 1, 4, 5, 2, 3

• b.) 1, 4, 3, 5, 2

• c.) 1, 2, 5, 3, 4

• d.) 1, 2, 3, 4, 5

20.) Which of the following correctly describes vaccines?

• a.) They are not considered medical interventions.

• b.) They cause the body to make more antigens

• c.) They cause the body to make antibodies against the antigen

• d.) They cause a mutation in the vaccinated person that makes them immune to the pathogen.

If 1 mL sample is put into tube #1 that has 24 ml diluent, what is the tube dilution?

• 1/25

• 1/50

• 1/24

• 1/625

If 1 mL sample from tube #1 with a tube dilution of 1/25 is placed in tube #2 with 24 mL of diluent, what is the final dilution of tube #2?

• final dilution 1/24 x 1/24

• final dilution 1/25

• final dilution 1/25 x 1/25

• final dilution 1/24

If 20 uL sample is put into tube #1 with 100 uL diluent, what is the tube dilution of tube #1?

• tube dilution 1/120

• tube dilution 20/120 or 1/6

• tube dilution 1/100

• final dilution 1/20