GFP Lab Mod 2/3 Quiz

Which term is closest in meaning to lysis?

• rupture

• isolate

• separate

• express

• grow

It is important to choose vividly glowing colonies for sub-culturing because they are most actively _______ GFP.

• translating

• transcribing

• secreting

• lysing

If you don't have a vortex machine, the next best way to mix contents of a small tube is to

• pipette up and down

• spin your body really fast while holding the tube

• invert it several times

• centrifuge the tube

In addition to the chemical action of lysis buffer, you will also do what to the cells to help break them open?

• freeze and thaw

• centrifuge

• put in a water bath

• shine a UV light on them

The liquid you obtain after you have broken the cells open is called

• lysate

• buffer

• cytoplasm

• GFP

Column chromatography will separate the cellular proteins according to

• size

• charge

• shape

• fluorescence

The process in which bacteria takes up exogenous DNA from the surrounding environment is known as

• transformation

• conjugation

• transduction

• recombination

When a protein is denatured, it has become

• unfolded

• globular

• digested

• unnatural

What is the name for the buffer that you will run through the chromatography column?

• elution

• wash

• lysis

• buffy

The chromatography column will slowly release liquid, drop-by-drop. How will you know that you have a drop containing GFP?

• it will come out first

• it will come out last

• it will glow under UV light

• it will appear yellow