MI U4 GFP Lab Mod 4 Pre-Lab

Electrophoresis separates molecules based on their relative

• size

• sequence

• shape

• function

How will you denature the proteins?

• boil them

• scare them

• apply electricity

• use lysis buffer

Will denatured GFP glow under UV light?

• yes

• no

• Maybe (Don't pick this)

Tags

NGSS.HS-PS4-3

What does the P in SDS-PAGE stand for?

• polyacrylamide

• phoresis

• protein

• pipet

What color is the stain we will use on the finished gels?

• blue

• green

• yellow

• pink

• black

Which TWO samples will we run through the electrophoresis gel? (Choose two!)

• GFP denatured

• GFP native

• GFP eluted

• GFP fluoresced

• GFP gritty frog people

What type of tube will you use when boiling protein samples?

• flip-top tubes

• screw-top tubes

• conical tubes

• glass test tubes

The gels you will use are held within a plastic "cassette", like a sandwich of plastic-gel-plastic.

• true

• false

The gels are very delicate and must be handled with extreme care during and after removal form the cassette.

• true

• false

Staining the gels is a one-step process. Just cover with stain and then drain the excess.

• true

• false