Analysis of nanostructures with microscopy

Increase of the diameter of the pinhole of a laser scanning microscope causes:

• decrease (deterioration) of spatial resolution

• growth of the field of view

• increase (improving) the spectral resolution

• increase of the level of the signal perceived by the photodetector

What kind(s) of light sources are used to excite fluorescence in conventional microscopy?

• xenon lamp

• mercury lamp

• incandescent lamp

• Halogen lamp

• Light-emitting diode

To allow the immersion lens operates correctly, it is necessary to have ... between the last lens and the object:

• Oil

• Air

• Water

• Nothing

What type of illumination is used in a confocal microscope in laser scanning mode?

• Ambient lighting

• Köhler method illumination

• Critical lighting

• Oblique lighting

What scheme of excitation and registration of fluorescence is used in the confocal microscope (LSM 710)?

• a

• b

• c

• d

The mark on the lens is 20x/0.75. What does it mean?

• Magnification 20x, field of view 0.75 mm

• Magnification 20x, aperture 0.75

• Resolution 20 Airy units, aperture 0.75

• Magnification 20x, working distance 0.75 cm

When using the dark field method in transmitted light, only ... reaches the objective lens.

• light passing through the sample without deflection

• light scattered on the sample

• luminescence

• light reflected from the sample

What affects the spatial resolution of a microscope?

• Lens aperture

• Lens magnification

• Refractive index of a medium between lens and object

• Wavelength

• All of the above

If we know that the sample absorbs in the 450-500 nm wavelength range with a maximum fluorescence at 530 nm, which set of excitation (EX) and emission (EM) broadband filters would you choose?

• EX 325 nm - 375 nm; EM 415 nm and above

• EX 445 nm - 495 nm; EM 520 nm and above

• EX 390 nm - 440 nm; EM 475 nm - and above

The dark field method as a visualisation method is used when

• Object is not transparent

• The object has low contrast

• fluorescent object

• None of the above