SDS-Page Protein Electrophoresis

Place ______ul of Native Protein Solution into tube labeled GFP Native

• 5

• 10

• 15

• 20

Add ____ul of protein denaturing solution to the tube labeled "GFP denatured."

• 5

• 10

• 15

• 20

Push bottom of GFP sample tube to be denatured through the foil on top of a beaker of boiling water and boil for _______ minutes.

• 10

• 2

• 4

• 5

Allow boiled (denatured) protein to _____ for a few minutes at room temperature.

• heat

• warm

• equilibrate

• cool

You will know that your protein has been denatured if it __________.

• glows under UV light

• does not glow under uv light

Before placing the cassette into the gel chamber, you must _______.

• remove the comb

• add a comb

• pipette your samples

For Edvotek gels, the shorter side of the plate of the cassette should face ____.

• the exterior

• the interior

After inserting the cassette into the gel chamber, cover the top of the cassette with _______.

• water

• LB Broth

• Buffer

• Salt water

You must _____ each well by squirting buffer into the wells using a transfer pipet to eject any air bubbles.

• melt

• rinse

• boil

• cool

Each gel will include a __________ in the first well.

• Protein sample

• Standard Protein Marker

• Guiding Dye

• DNA Ladder