Recombinant DNA

A cell is made up almost entirely of _______________. 

• Carbohydrates

• Proteins

• fats

• nucleic acids

A protein of interest is _____________. 

• the entire genome of the organism

• a section of DNA (a gene) that codes for a protein

• a protein that can be used to treat a disease

• a protein that has an interesting shape

Which of the following is a circular DNA from bacteria that can hold a foreign gene?

• plasmid

• restriction enzyme

• ligase

• protein expression

Which of the following is a protein that acts as DNA scissors?

• plasmid

• restriction enzyme

• ligase

• protein expression

Which of the following is an enzymatic protein that acts as DNA glue?

• plasmid

• restriction enzyme

• ligase

• protein expression

Which is true about plasmids?

• Plasmids can be cut at specific sequences called restriction sites.

• Plasmids contain a promoter sequence that defines where transcription begins.

• Plasmids can contain an antibiotic resistance gene.

• All of the statements are true about plasmids.

Why are plasmids sometimes given an antibiotic resistance gene?

• So that scientists can make antibiotic resistant bacteria and make more money for pharmaceutical companies.

• So that scientists can create super bugs.

• So that scientists can identify which cells have the rDNA plasmid.

• So that scientists can kill the bacteria.

In bacteria, restriction enzymes are used for defense. They cut up the DNA of a virus that infects bacteria.

• TRUE

• FALSE

Once the gene and plasmid have been cut with a restriction enzyme, another enzyme called _________ is used to join the pieces of DNA together.

• polymerase

• ligase

• dehydrogenase

• lactase

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• rDNA technology

• transference

• transformation

• rDNA plasmid