Synthetic Biology Review

Which genetic engineering technique amplifies the number of DNA pieces in a lab setting?

• PCR

• Gel Electrophoresis

• Gene Therapy

• CRISPR

Which genetic engineering technique separates DNA fragments based on their size?

• PCR

• Gel Electrophoresis

• Gene Cloning

• CRISPR

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Which is the primary purpose of using restriction enzymes in gel electrophoresis?

• It allows the strands of DNA to be cut into various lengths for testing
• It restricts the number of base pairs that can be tested in a sample
• It makes the testing simpler by moving the strands into the gel faster
• It charges the DNA strands

The main goal of the Human Genome Project (HGP) was to:

• Identify the unique parts of each person's DNA.
• Identify genes responsible for human diseases.
• Identify genes responsible for human behavior.
• Determine the entire sequence of human DNA.

In order to insert a human gene into plasmid, both must 

• code for the same gene product.
• be cut by the same restriction enzyme.
• originate from the same type of cell.
• have identical sequences.

An enzyme that cut DNA molecules at a specific “recognition site” is called

• Restriction enzyme

• DNA polymerase

• RNA polymerase

• Taq polymerase

Which suspect’s DNA matches the felon’s DNA left at the crime scene?

• Suspect 1

• Suspect 2

• Suspect 3

Tags

NGSS.HS-LS3-2

The parents of a new baby believe they brought the wrong child home from the hospital. Gel electrophoresis was performed using DNA samples from the parents and the child. A section of the gel

electrophoresis results is shown below. Which conclusion is valid based on the gel electrophoresis results?

• They have the correct child, because her genetic information is identical to that of the father.

• They have the wrong child, because her genetic information does not match that of either parent.

• They have the correct child, because her genetic information matches the genetic information from both parents.

• They have the wrong child, because her genetic information matches only that of the mother.

Tags

NGSS.HS-LS3-1

NGSS.HS-LS3-2