7.1 Methods in Gene Cloning

First stage involved in gene cloning.

• Amplification of the target gene

• Insertion of the target gene

• Polymerase Chain Reaction

• Isolation of the target gene

The plasmids carrying the target gene (recombinant plasmids) must be introduced into a host cell through _____.

• Cloning

• insertion

• Transformation

• Isolation

Types of plasmids produced after insertion of the target gene into a vector.

• Recombinant plasmids

• Non-recombinant plasmids

• Bacterial plasmids

• Isolated plasmids

Which technology below would probably be the most important to a person who had diabetes and had to take insulin every day?

• using recombinant DNA to produce human hormones from bacteria

• testing parents for genetic disorders before they have children

• engineering fruits and vegetables that resist insects and other pests

• C. developing ways to identify criminals through DNA fingerprinting

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NGSS.HS-ETS1-3

How do scientists cut DNA into smaller strands?

• restriction enzymes

• scissors

• ligase

• agarose

In order to insert a human gene into plasmid, both must

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.

Which enzyme "glues" DNA fragments together during insertion?

• restriction enzymes

• DNA ligase

• DNA polymerase

• DNA helicase

_________ DNA is created by using the ______ from a bacteria and splicing a section of DNA into it.

• recombinant, plasmid
• Cloning, ribosome
• transcription, translation
• cumulus, mitochondria

Which of the following could be used as a vector for a human gene?

• Bacterial Plasmid

• Bacteria

• A mitochondria

• mRNA

The site where restriction enzymes cut are a called:

• cut sites

• restriction sites

• recognition sites

• cleave sites