Flow Cytometry

What is the main purpose of Flow Cytometry 

• It's used to count and analyze the size, shape, and properties of a heterogeneous mixture of cells

• It's used to measure the IR absorption of a homogeneous mixture of cells

  cells

• It's used to use to sort and separate cells by their size and mass

• It's used to quantify the transmittance light when a laser is shown through a mixture of cells

What is the system that moves particles in the FC

• Electronic system

• Hydraulics system

• Fluidic system

• Optical system

What is the main principle of Flow Cytometry

• The measurement of the cell's ability to withstand high pressures

• The measurement of molecular vibrations produced by the cell population when absorbing electromagnetic radiation

• The measurement of the separation between a heterogenous cell mixture bombarded with heat

• The measurement of light scattered by individual cells, and the fluorescence observed from them

What is Forward-scattered light (FSC) a measurement of?

• It's a measurement of diffracted light 

• Its a measurement of the diffraction index of the cell population

• Its a measurement of the transmittance of light that penetrates the cell population in solution

• Its a measurement of the absorption of the cell population

What is Side-scattered light (SSC) a measurement of?

• Its a measurement of the refraction index of the cell population

• It's a measurement of diffracted light 

• It's a measurement of refracted and reflected light

• Its a measurement of absorbed light

What transports the sample cells through the flow cytometer into the path of the laser

• High-pressure Inert Gas

• Low-Pressure Inert Gas

• A sheath fluid medium 

• Non-Volatile Polar solvents

How many fluorescence detectors are in the sample flow cytometer

• Three

• None

• Six

• There are phosphorescence detectors

What type of detectors are used in flow cytometry 

• Infrared

• Fluorescence

• X-ray 

•  UV

What type of information conversion occurs in the Electronics System of the flow cytometer

• Digital to Time

• Frequency to Analog

• Analog to Digital 

• Time to Digital