Paper Chromatography

What is Paper Chromatography?

• A technique used to separate components in a dye based upon molecules having different polarities.

• A technique used to combine different pigments together to obtain one color substance

• A technique used to isolate different constituents based upon their color

• A technique used to add color to neutral substances that can dissolve in alcohol

When using paper chromatography, two phases are involved. They are the

• stationary and mobile phases

• solid phase and liquid phase

• gaseous phase and mobile phase

• constituent phases and analyte phase

Based upon the results of a chromatography done on three different black inks, which ink was composed of only 2 constituents?

• A

• B

• C

• D

Tags

NGSS.HS-PS1-3

Assuming isopropyl alcohol was used, which constituent was the most polar for sample C?

• blue

• violet

• yellow

• black

Why in paper chromatography does one not allow the solvent to migrate totally up the paper to the top edge of the chromatography paper?

• All constituents would recombine into one color

• the mixture could explode

• the mixture could evaporate off the edge of the chromatography paper

• the solvent might dilute the constituents

To calculate the R_f or retention factor, one divides the distance the _______ moved by the distance the ______ travelled.

• spot; solvent

• solvent; spot

• paper; solvent

• spot; paper

Spinach leaves were used to extract chlorophyll from the leaves. Then a paper chromatography was conducted on the analyte giving the following results. What can be concluded about the chlorophyll in spinach?

• There are four constituents in chlorophyll

• spinach should be yellow instead of green

• the chlorophyll indicates that the spinach was ripe

• the leaves did not get enough sunlight during daytime hours

Tags

NGSS.HS-LS1-5

NGSS.HS-LS2-5