WESTERN BLOT

A student researcher overexpresses an exogenous protein in cell culture and wants to determine if that protein, is in fact, overexpressed. What technique would best demonstrate that this protein is expressed in these cells?

• NORTHERN BLOT

• SOUTHERN BLOT

• WESTERN BLOT

• EASTERN BLOT

Which of the following probes are most commonly used in Western blotting?

• NUCLEIC ACIDS

• ANTIBODIES

• BOVINE SERUM ALBUMIN

• ALL OF THE ABOVE

What is the purpose of "blocking" the nitrocellulose membrane during western blotting?

• To encourage non-specific binding

• To improve binding of secondary antibody

• To prevent non-specific binding

• Scientists like extra work

What is the purpose of washing in every step in western blotting?

• to ensure a stable pH environment in the tank

• to remove any unbound antibodies

• to remove any contaminating residues

• to avoid protein degradation

• to moisten the transfer membrane

STATE THE PURPOSE OF BLOCKING

It is a small piece of DNA used to find another piece of DNA in blotting.

It maintains polypeptides in a denatured state once they have been treated with strong reducing agents to remove secondary and tertiary structure.

What do you call to the technique in which nucleic acids or proteins are immobilized onto a solid support?

Western blot is a method used to check for the presence of?

What is the first step in blotting technique?

• Detection and analysis of molecules of interest

• Separation of molecules based on size and charge

• Transfer of separated molecules from a gel to a membrane

• Labeling of probes or antibodies