Serological and Molecular Detection of Bacerial Infections

- host and microbes live together long term

- indigenous microbiota

• symbiotic

• commensalistic

• mutualistic

• parasitic

- no benefit or harm to either organism

• symbiotic

• commensalistic

• mutualistic

• parasitic

- both host and microbes benefit

• symbiotic

• commensalistic

• mutualistic

• parasitic

- microbes cause harm to the host

• symbiotic

• commensalistic

• mutualistic

• parasitic

organism's ability to establish an infection

• infectivity

• pathogenicity

• virulence

ability of an organism to cause disease

• infectivity

• pathogenicity

• virulence

extent of pathology caused by an organism when it infects a host

• infectivity

• pathogenicity

• virulence

- Growth on broth or solid media

- Major means of diagnosis, but may take time or may not be possible

• culture of the causative agent

• microscopic examintaion

• detection of bacterial antigens

• molecular detection of bacterial DNA or RNA

• serology

- gram stain or special stain

• culture of the causative agent

• microscopic examintaion

• detection of bacterial antigens

• molecular detection of bacterial DNA or RNA

• serology

- rapid testing by ELISA, LFA, or LA

• culture of the causative agent

• microscopic examintaion

• detection of bacterial antigens

• molecular detection of bacterial DNA or RNA

• serology