Quiz Molecular Biology

The alternative selectable marker _______________ and chromogenic substrate __________________ that selects recombinants on the basis of their ability to produce colour

• LacZ gene ; X-gal

• IPTG ; β-galactosidase

• Amp Resistance gene ; X-gal

• β-galactosidase ; IPTG

__________ is also known as "light" chromatin and represents DNA that can actively undergoing __________.

• Heterochromatin, translation

• Euchromatin, translation

• Heterochromatin, transcription

• Euchromatin, transcription

This notable figure is the one that discover DNA component in the pus of wounded soldier’s bandages

• Thomas Morgan

• Friedrich Miescher

• Phoebus Levene

• Thomas Avery

Name the histone protein that used to link between nucleosomes

• H2A, H2B

• H3

• H4

• H1

Identify the statement that is not true regarding restriction enzymes used in recombinant DNA technology.

• They are site-specific

• They recognize palindromic sequences

• They are endonucleases

• They are ATP dependent nucleases

Identify the false fact about nucleases

• Nucleases catalyze the hydrolytic cleavage of the phosphodiester linkages.

• Nucleases can be exonuclease and endonucleases.

• Exonucleases cleave the sequence by staggered cutting

• Exonucleases cleave the chain randomly between any nucleotides.

Non-essential genes/stuffer fragments are removed from bacteriophage because

• To reduce the phage genomic RNA size

• To increase the phage genomic DNA size

• To fit in other phage DNA

• To fit in the foreign gene to be cloned

Cosmid is a hybrid between _________ and _____________ because ______________ .

• phage, plasmid, they are packaged into a λ phage head but they replicate as plasmid in bacteria

• cosmid, plasmid, they behave like phage by infecting bacteria but replicate in phage head

• phage, plasmid, they behave like phage by infecting bacteria but replicate in phage head

• bacteria, plasmid, they are packaged into a λ phage head but they replicate as plasmid in bacteria

What is the capacity of cosmid vector?

• 10-12 kb

• 20 kb

• 45-50 kb

• 300kb

Statement below is true, EXCEPT:

• Mendel’s shows the genes can exist in several different forms, or alleles.

• Morgan provided the first definitive evidence for the chromosome theory using Drosophila melanogaster as experimental subject.

• Watson and Crick proposed the x-ray diffraction data to map the DNA models based on chemical and physical data.

• McClintock shown a physical demonstration of recombination in maize chromosomes.

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