DNA Cloning & Recombinant DNA Technology

Define recombinant DNA technology

• A method in which same DNA from same organisms is spliced (combine) together in the laboratory

• A method in which DNA is spliced together in the laboratory

• A method in which different DNA from same organism is spliced (combine) together in the laboratory

• A method in which DNA from different organisms is combined together in the laboratory

Which of the following is correct sequence of process in recombinant DNA technology?

1. 1. Isolation of target DNA & vector

2. 2. Introducing recombinant DNA into host cell

3. 3. Fragmentation of target DNA & vector by restriction endonuclease

4. 4. Ligation of DNA fragment into vector

5. 5. Culturing in host cell to get desired products.

• 1,2,3,4,5

• 1,3,4,2,5

• 1,2,4,2,5

• 1,4,3,2,5

Which of the following terms describes the process of the uptake of recombinant plasmids into bacteria cells?

• amplification

• insertion

• transformation

• screening

In order to insert a human gene into plasmid, both must

• code for the same gene product.

• be cut by the same restriction enzyme.

• originate from the same type of cell.

• have identical sequences.

A cloning vector is

• An agent that transfers a pathogen

• An agent that transfers a foreign gene from one DNA molecule to another

• An agent that transfers a DNA segment to a RNA molecule

• An agent that transfers a RNA segment to a DNA molecule

Which one or more of these can be used as a vector?

i. Bacterium ii. Plasmid iii. E. coli iv. Bacteriophage

• i & ii

• ii & iii

• i & iii

• ii & iv

Which of the following is CORRECT about host cell?

• Plasmid is an example of host cell.

• Unable to receive DNA molecule through transformation.

• An organism that prevents cloning vectors from multiplying.

• An organism that is used to receive recombinant DNA for cloning purposes. 

DNA manipulative enzymes can be grouped into four broad classes, depending on the type of reaction that they catalyse:

1) Nucleases: cut, shorten, or degrade nucleic acid molecules.

2) Ligases: join nucleic acid molecules together.

3) Polymerases: make copies of molecules.

4) Modifying enzymes: remove or add chemical groups.

• True

• False

DNA polymerases are enzymes that synthesize a new strand of DNA complementary to an existing DNA or RNA template.

Which of the following is involved in PCR?

• • DNA polymerase I

• • Klenow fragment

• • Taq DNA polymerase

• • Reverse transcriptase

Answer explanation

• DNA polymerase I processes RNA primers during lagging-strand synthesis and fills small gaps during DNA repair reactions.

• Klenow Fragment is the large fragment of DNA Polymerase I, a modified enzyme that retains the polymerase function but is unable to degrade DNA

• Reverse transcriptase, an enzyme involved in the replication of several kinds of virus. Reverse transcriptase is unique in that it uses as a template not DNA but RNA.

Which of the following enzymes can cut nucleic acids?

• DNA polymerase

• restriction endonuclease

• modifying enzymes

• reverse transcriptase