MolBio Lec Finals-Lesson 13: DNA Sequencing

MolBio Lec Finals-Lesson 13: DNA Sequencing

University

17 Qs

quiz-placeholder

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MolBio Lec Finals-Lesson 13: DNA Sequencing

MolBio Lec Finals-Lesson 13: DNA Sequencing

Assessment

Quiz

Biology

University

Hard

Created by

John Infante

Used 2+ times

FREE Resource

17 questions

Show all answers

1.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

Frederick Sanger won Nobel Prize in 1980 for sequencing phage OX174. He was already awarded with Nobel Prize in 1958 for
discovery of sanger's method
elucidating amino acid sequence of insulin
elucidating nucleotide sequence of insulin
elucidating nucleotide sequence of stomatostatin

2.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

Automated DNA sequencing is an improvement of Sanger's method method where
ddNTPS are used for chain termination
Fluorescently labelled dNTPs are used for chain termination
Fluorescently labelled ddNTPs are used for chain termination
PCR is used for making sequencing templates

3.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

The chemical used for strand cleavage in Maxam-Gilbert method is
Piperidine
Hydrazine
Formic acid
DMSO

4.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

The Maxam-Gilbert method, chemical used for cytosine alteration is
formic acid
hydrazine
dimethyl sulphate
piperidine

5.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

In automated DNA sequencing,
radiolabeled dNTPs are used
radiolabeled ddNTPs are used
fluorescently labeled dNTPs are used
fluorescently labeled ddNTPs are used

6.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

How many ddNTPs are used in Sanger's method?
1
2
3
4

7.

MULTIPLE CHOICE QUESTION

1 min • 1 pt

The primer used in Sanger sequencing
can have any nucleotide sequence
must have a sequence beginning and ending with the same nucleotide
has a nucleotide sequence complementary to the 3' end of the region to be copied
has a nucleotide sequence complementary to the 5' end of the region to be copied

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