Bio2581B - Tutorial 3 practice Quiz from synchronous sessions

There are three RNA-mediated methods that can be used to modify gene expression patterns. Which protein listed below is not associated with any RNA-mediated gene expression control mechanism?

• RNAase
• AGO 2 protein
• RNA pol II
• Cas 9
• RNA pol I

What is the correct order of steps for the CRISPR process?​

• binding, targeting, repairing, cleaving​

• targeting, binding, cleaving, repairing​

• cleaving,

  binding, targeting, repairing​

• targeting, cleaving, binding, repairing​

• targeting, binding, repairing, cleaving​

You do a complementation analysis on 2 haploid mutant alleles wing1 and wing2. It is found that a diploid organism is wing- and therefore, has no wings. Based off of this information, these 2 haploid alleles show:

• a) Complementation and are in the same complementation group

• b) Non-complementation and are in the same complementation group

• c) Complementation and are in different complementation groups

• d) Non-complementation and are in different complementation groups

• e) Both complementation and non-complementation

Which tool allows you to determine where a peptide is localized in a cell?

• A. Ensembl
• B. Xpasy ​
• C. UniProt ​
• D. BLAST
• E. CRISPR

What is the difference between forward and reverse genetics?

• Forward genetics uses a genotype to phenotype approach while reverse uses a phenotype to genotype approach.

• Forward genetics uses a phenotype to genotype approach while reverse uses a genotype to phenotype approach.

• forward genetics, reintroducing DNA is necessary, but is not needed when doing reverse genetics.

• When performing forward genetics you should ask “what process is the gene important for” and when doing reverse genetics you should ask “what genes are important for a certain process”

• There is no difference between forward and reverse genetics.

see image for question

• Forward genetic, Phenotype to Genotype, 1

• Forward genetic, Genotype to Phenotype, 2

• Forward genetic, Phenotype to Genotype, 2

• Reverse genetic, Genotype to Phenotype, 2

• Reverse genetic, Phenotype to Genotype, 1

Joe has a mouse with fur pigmentation that is mottled black and brown. What epigenetic phenomena might this scenario belong to?

• a) Imprinting where the expression of the patchy colouration gene depends on DNA methylation

• b) X inactivation and this is probably a male mouse with a black allele on the X and a brown allele on the Y

• c) RNA epigenetics where miRNA is regulating the patchy colouration gene

• d) X inactivation and this is a female mouse with a black allele on one X and a brown allele on the other X

Solenopis geminate is a species of fire ant native to Texas. One day you were walking in Dallas and found newly born ants with mutations that have no eyes. You decide to take the mother ant (who has normal eyes) to test it further. When mated with a wildtype male ant, the mother seems to lay normal eggs, however, when the fire ants are born they all have no eyes. A mutation in what kind of gene is likely driving this effect?

• Maternal effect gene

• Zygotic effect gene

• Gap gene

• Homeotic gene

• None of the above

Which of the following would prevent RNAi from degrading mRNA?

• RNAPII produces a secondary miRNA transcript
• Inhibition of DNA polymerase I
• Homozygous loss of function mutation to AGO, preventing mRNA recognition
• A heterozygous gain of function mutation to dicer, upregulating digestion
• miRNAs stop translation and degrade the transcript