PCR & Applications

The PCR technique was developed by

• Karry Mullis

• Kohler

• Milstein

• Altman

PCR is a

• DNA Sequencing technique

• DNA Amplification technique

• DNA Degradation technique

• All of these

Which of the following statements are true regarding PCR

• Automated PCR machines are called thermal cyclers

• A thermostable DNA Polymerase is required

• Billion copies of desired DNA can be synthesized from microgram quantities of DNA

• All of these

Thermus aquaticus is the source of

• Primase enzyme

• Taq Polymerase

• Vent Polymerase

• Both 2 and 3

All of the following are thermostable polymerases except

• Taq Polymerase

• Vent Polymerase

• DNA Polymerase III

• pfu Polymerase

the basic requirements of PCR reaction include

• DNA segment to be amplified

• Two oligonucleotide primers

• a heat stable DNA polymerase

• all of these

The first step in PCR is

• Primer annealing

• Denaturation

• Primer extension

• None of these

Denaturation involves

• heating between 40-60 C

• heating between 90-98 C

• heating between 70-72 C

• None of these

Reverse transcription PCR uses

• mRNA as a template to form

• RNA as a template to form a DNA

• DNA as a template to form ssDNA

• all of these

The process represented in the figure is known as

• RAPD

• Southern Blotting

• Northern Blotting

• PCR Amplification