Micro lec 3

Match the following

• Disinfectant

• Destroys all pathogens; spores, vegetative cells, fungi, and viruses

• Personal Protective Equipment (PPE)

• Disposable gloves, laboratory gowns, face masks, and eye protection

• Antiseptic

• Destroys or inactivates bacteria, viruses, and fungi (not spores)

• Sterilization

• Chemical germicide used on skin

Match the following

• Antiseptics

• 70% ethyl or isopropyl alcohol

  Iodophors

  Triclosan

• Disinfectants

• 70% ethanol or 10% bleach solution used to decontaminate benchtops.

  Phenolics – additive to detergents

• Sterilization

• Steam Autoclave

  121°C for 1 hour at 15 psi of pressure

Match the following

• •BSL 1

• •No knowns pathogenic potential

  •Adhere to standard lab techniques

• •BSL 2

• Level 3 practice, plus entrance through separate room which street clothing is replaced with lab clothing. Maximum containment. Decontamination of all personnel and material before leaving area

• •BSL 3

• •Level 1 practices, plus lab coat, gloves, decontamination of infectious waste, biohazard warning signs

• •BSL 4

• •Level 2 practices, plus special lab clothing, controlled access

  •Air movement carefully controlled

Match the following

• BSC I

• Open-fronted, negative-pressure, ventilated cabinet

  Unsterilized air

  Have HEPA filter

• BSC II

• Used for any procedures that create aerosols.

  Classified as BSC I, II, or III 

• Biological Safety Cabinets (BSC)

• Open-fronted, ventilated cabinet

  Sterilizes air entering and circulating withing cabinet and exhaust air

  Have HEPA filter

• BSC III

• Highest level of safety

Place The PPE in the squares.

e

d

b

a

Disposable Gloves

Laboratory gown

Face mask

Eye protection

Sandels

Cloth gloves

Put the organisms in order from most resistant to least resistant.

• Prions, Bacterial spores, Mycobacteria, Nonlipid Viruses, Fungi, Bacteria, Lipid viruses

• Prions, Lipid viruses, Bacteria, Fungi, Nonlipid viruses, Mycobacteria, Bacterial spores

• Bacterial spores, Nonlipid viruses, Fungi, Bacteria, Mycobacteria, Prions, Lipid viruses

• Bacterial spores, Fungi, Lipid viruses, Bacteria, Mycobacteria, Prions, Nonlipid viruses