HPLC method development

Below are true about chromatographic technique, except

• it can separate a complex mixture

• it can identify compounds exist if standard of compounds provided

• it can quantify constituent(s)

• it provide information about functional groups exist in a sample

Referring to the picture of HPLC components, what is D?

• Pump

• Detector

• Monitor

• Injector

What does HPLC stand for?

• High-Performance Laboratory Chromatography

• High-Pressure Liquid Chromatography

• High-Performance Liquid Chromatography

• High-Resolution Liquid Chromatography

Which of the following is NOT a component of an HPLC system?

• Detector

• Injector

• Centrifuge

• Column

Which type of HPLC column packing material is most commonly used for reversed-phase chromatography?

• Paper

• Silica gel

• Polystyrene

• Octadecylsilane (C18)

In HPLC, the mobile phase is also known as:

• Eluent

• Solvent

• Detector

• Column

Which parameter is primarily adjusted during the optimization of an HPLC method to separate compounds efficiently?

• Flow rate

• Detector wavelength

• Mobile phase composition

• Injection volume

Which type of detector is commonly used for UV-Vis detection in HPLC?

• Mass spectrometer

• Refractive index detector

• Fluorescence detector

• PDA detector

What is the purpose of the mobile phase gradient in gradient elution HPLC?

• To increase the column temperature

• To maintain a constant mobile phase composition

• To change the mobile phase composition during the analysis

• To decrease the detector sensitivity