What is the function of the ocular lens in a compound microscope?

To direct the light and magnify the image

To separate small objects that are close together

To choose the desired magnification

Why do higher power objectives have shorter working distances?

To allow more light to pass through the objective lens

What is the purpose of using oil immersion with the objective lens?

To improve the resolution and clarity of the image

To increase the working distance

What is the proper method for discarding used gloves at the end of the lab?

Throw them in the regular trash bin

Dispose of them in a biohazard bucket if contaminated

What is the effect on field of vision as magnification increases?

Field of vision becomes smaller

Field of vision remains unaffected by magnification

Field of vision becomes blurred

When increasing magnification, what are two ways to adjust the amount of light shining through the specimen?

Rotating the condenser adjustment knob.

Removing the iris diaphragm lever.

Increasing the voltage of the microscope.

How can the amount of light shining via a specimen be adjusted when increasing magnification?

Rotate the light intensity knob

Adjust the iris diaphragm lever

Before switching to the 100x objective, what should be done to ensure visibility of bacteria at increased magnification?

Move the slide to position bacteria in the center view

Refocus using the coarse focus knob on the area with bacteria

If very few organisms are present in a dilute bacterial culture viewed at 40X objective, what should be done before switching to 100X objective?

Move slide to bring area with most visible bacteria to center view

Increase light intensity using the condenser adjustment

Reduce light intensity using iris diaphragm lever

Clean microscope lens thoroughly

What steps should be taken once finished using the microscope?

Return objective lens, remove slide, clean lens, turn off microscope, unplug and store cables/cords, store microscope in cabinet with bag cover

Remove slide, turn off microscope, unplug and store cables/cords, store microscope in cabinet without bag cover

Leave everything as is and walk away from the microscope

Microbiology - Lab 1

Authored by df cv

Biology

University

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