JC231009

Do you like the pizzas?

• Yes, they're perfect!

• Yes, but we need more pizza!

• No, too little cheese

• No, I don't like pizza

Are the mutants comparable enough to their respective wild-type (in terms of general health parameters) for studying virus transmission?

• Yes, the effects on health alone should not impact infection metrics

• Yes, but we must keep in mind that the mutants are not 100% healthy

• No, the impact on health is too great for studying virus transmission

• I have two different opinions for the two different mutants

Is PFU per whole mosquito (as reported for the Ago2 mutant) a valuable metric?

• Yes, but very blunt and requires organ-specific investigation

• No, it is useless because it says nothing about where the virus is

• Yes, it is representative of the mosquito's sensitivity to infection

• No, you will never know a mosquito unless you dissect it

Why do you think that knocking-out Dicer2 did not affect transmission?

• Dicer2 is not impactful enough in this genetic background

• Dicer2 is only important in the early stages of infection

• A larger sample size is needed to demonstrate the effect

• Dicer2 is not impactful enough for the virus strains tested

• I have a different opinion

Why do you think the Ago2 mutant mosquitoes die faster when infected?

• They have a reduced tolerance for the virus

• They are inherently sick and cannot sustain an infection

• The viral load overwhelms the mosquito

• I have a different opinion

Why do you think the Dicer2 KO mosquitoes die faster when infected with MAYV?

• They have a reduced tolerance for the virus

• They are inherently sick and cannot sustain an infection

• The viral load overwhelms the mosquito

• I have a different opinion

Do you think that the 21nt fragments detected in the Dicer2 KO could function as siRNAs?

• Yes, these fragments show that the mutant is incomplete

• No, these are just degradation products that serve no purpose

• Yes, a fragment could guide RISC even if it is not produced by Dicer2

• I have a different opinion

Did you like this format for the journal club?

• Yes, perfect!

• No, I have strong opinions.

• Yes, but I still have opinions.

Which date is best for you for the next journal club ?

• 2023/11/09

• 2023/11/10

• 2023/11/16

• 2023/11/20