19GT (CIE A-Level)

Circle DNA polymerase on this diagram

Answer explanation

When creating cDNA from mRNA, the first enzyme used is reverse transcriptase. The second enzymes used is DNA polymerase.

During PCR _______ anneal to complementary bases at the ends of the sequence to be replicated

During gel electrophoresis, DNA moved towards the _____

Circle where DNA polymerase binds to this plasmid

Name the technique used to amplify DNA

• Gel electrophoresis

• Alcohol precipitation

• PCR

• Microarray

Name the technique used to investigate gene expression

• Gel electrophoresis

• Alcohol precipitation

• PCR

• Microarray

Name the technique used to separate DNA fragments by length

• Gel electrophoresis

• Alcohol precipitation

• PCR

• Microarray

______ genes are used to help identify successful transformants

Calcium chloride and ____ _____ is used to improve transformational efficiency.

Which step of PCR takes place at 95C

• Denaturation

• Annealing

• Hybridisation

• Elongation