Unit 11

Which colony plating method is used for the separation into pure culture of organisms from mixed culture?

• Streak Plate

• Pour Plate

• Spread Plate

• Both Streak Plate and Pour Plate

What is the purpose of streaking the agar plate in the streak plate method?

• To dilute the bacteria

• To count the number of colonies

• To identify the bacteria

• To solidify the agar

How are specific bacterial cells isolated in the streak plate method?

• By pouring the liquid sample in the center of a Petri dish

• By spreading the diluted culture on the agar surface

• By streaking the inoculum over successive quadrants

• By mixing the sample with the molten agar medium

What is the purpose of incubating the streak plates at 37°C for 24 hours?

• To examine the colonies for growth

• To identify the bacteria

• To separate the bacterial cell colony

• To obtain a pure culture

Which colony plating method is commonly used for isolation of bacteria?

• Streak Plate

• Pour Plate

• Spread Plate

• Both Streak Plate and Pour Plate

What is the purpose of the pour plate method?

• To dilute the bacteria

• To count the number of colonies

• To separate the bacterial cell colony

• To identify the bacteria

How is the inoculum added in the pour plate method?

• By streaking the inoculum over successive quadrants

• By pouring the liquid sample in the center of a Petri dish

• By spreading the diluted culture on the agar surface

• By mixing the sample with the molten agar medium

What is the purpose of inverting the agar plate in the pour plate method?

• To examine the colonies for growth

• To identify the bacteria

• To separate the bacterial cell colony

• To allow colonies to form throughout the plate

What is the advantage of the spread plate method?

• Allows the growth of microaerophilic bacteria

• Has a longer shelf life

• Requires a smaller inoculum size

• Provides even distribution of colonies

What is the disadvantage of the spread plate method?

• Does not allow the growth of microaerophilic bacteria

• Requires a larger inoculum size

• Has a limited shelf life

• Causes osmotic shock to the bacteria