Molecular Biology Techniques

What does PCR stand for?

• Protein Chain Reaction

• Polymerase Chain Replication

• Polymerase Chain Reagent

• Polymerase Chain Reaction

What is the purpose of gel electrophoresis in molecular biology?

• To amplify DNA sequences

• To determine the sequence of DNA

• To visualize DNA under a microscope

• To separate and analyze DNA, RNA, or proteins based on their size and charge.

What is the main technique used in DNA sequencing?

• Next-generation sequencing

• Polymerase chain reaction

• Gel electrophoresis

• Sanger sequencing

What is the process of creating a genetically identical copy of an organism called?

• replication

• mutation

• hybridization

• cloning

What are restriction enzymes used for in molecular biology?

• Cutting DNA at specific sequences

• Detecting DNA mutations

• Amplifying DNA sequences

• Joining DNA fragments together

Which enzyme is commonly used in PCR to amplify DNA?

• RNA polymerase

• DNA polymerase

• Taq polymerase

• Ligase

What is the purpose of loading dye in gel electrophoresis?

• To track the movement of DNA or RNA molecules and determine their size.

• To provide color to the gel

• To enhance the conductivity of the gel

• To prevent the gel from drying out

What is the Sanger sequencing method also known as?

• Polymerase chain reaction

• DNA microarray

• Chain termination method

• Next-generation sequencing

What is the vector commonly used in cloning experiments?

• chromosome

• protein

• plasmid

• gene

What is the function of restriction enzymes in molecular biology?

• Restriction enzymes join DNA at specific recognition sites.

• Restriction enzymes repair DNA at specific recognition sites.

• Restriction enzymes cut DNA at specific recognition sites.

• Restriction enzymes amplify DNA at specific recognition sites.