Micropipetting and Electrophoresis

In gel electrophoresis, the largest DNA fragment will appear

• closest to the starting wells
• farthest from the starting wells
• three quarters away from the starting wells
• it depends on how many fragments there are

Why do the fragments of DNA in gel electrophoresis travel away from the negative electrode?

• DNA is negatively charged so attracted to the positive end of the unit
• DNA is positively charged to attracted to the negative end of the unit
• the agarose gel in negatively charged
• the agarose gel is positively charged

Tags

NGSS.HS-PS2-5

NGSS.HS-PS3-5

Which suspect is likely guilty?

• Suspect #1
• Suspect #2
• Suspect #3

Based on these results whose blood was found in the blood stain at the crime scene?

• Bob
• Sue
• John
• Lisa

Tags

NGSS.HS-LS3-2

What is the purpose of gel electrophoresis?

• helps cut DNA
• count the genes in DNA
• separates DNA based on size
• allows for an exact replicated organism

What applications can gel electrophoresis be used for?

• Parental Testing
• Criminal Investigations
• Sequence Endangered Species DNA
• All of the above

A student performed a gel electrophoresis experiment. The results are represented in the diagram

below. Compared to the fragments at the top of the gel, the fragments at the lower end are

• larger, and move slower

• larger, and move faster

• smaller, and move faster

• smaller, and move slower

Tags

NGSS.HS-LS3-2

DNA fingerprinting works because

• Most genes are dominant
• All organisms contain RNA
• The most important genes are different among most people
• No two people, except identical twins, have exactly the same DNA 

When using a micropipettor, if you push to the second stop to fill it you will get ____________

• the correct amount of solution
• too much solution
• too little solution
• either too much or too little solution

The best tool to measure 146 uL (microliters) ______________.

• A PTP (plastic transfer pipette)
• A 5 - 50 uL micropipettor
• A 2 - 200 uL micropipettor
• A graduated cylinder