Microbial metabolism

Microbial metabolism

• life support processes that enable microbes to thrive

• sum of catabolic & anabolic reactions

• - release energy

• - breakdown of complex organic compounds into simpler ones

• - degradative reactions generally hydrolytic reaction

• - exergonic (produce > energy than consume)

• - e.g. Degradation of sugar into CO2 & H2O

• Catabolism

• Anabolism

• - use energy

• - building complex organic compounds from simpler ones

• - biosynthetic reactions : dehydration

  - endergonic (consume > energy than produce)

• - e.g. formation of proteins from aa

• Catabolism

• Anabolism

Coupling of Catabolic & Anabolic reactions

• catabolic reaction provide building blocks for anabolic reactions

• coupling of energy-requiring & energy-releasing reactions thru ATP mlcl

• ATP stores energy derive from catabolic r. that used to drive anabolic r.

Answer explanation

• true

• false

Enzymes

• Substances that accelerate chem. reactions by lowering down E_a

• large globular proteins folded in 1°, 2° or 3° configuration -> specificity

• able to catalyze reactions at rates 10⁸ to 10¹⁰ times higher than reactions w/out enzyme

• Turnover no. btwn 1-10k (can as high as 500k)

• active site on enzyme react w/ specific substrate

Answer explanation

Turnover no. : max no. of substrate an enzyme converts to product each second

• - [O] & [H]

• - cytochrome oxidase

• - lactate dehydrogenase

• Transferase

• Oxidoreductase

• Hydrolase

• Ligase

• Isomerase

• - transfer of f(x)al grp : amino grp, acetyl grp, phosphate grp

• - acetate kinase

• - alanine deaminase

• Transferase

• Oxidoreductase

• Hydrolase

• Ligase

• Isomerase

• - hydrolysis

• - lipase

• - sucrase

• Transferase

• Oxidoreductase

• Hydrolase

• Ligase

• Isomerase

• - removal of grps of atoms w/out hydrolysis

• - oxalate decarboxylase

• Transferase

• Lyase

• Hydrolase

• Ligase

• Isomerase