BIO 2134 Tutorial Nanostring and microscopy

Identify component "D" of the complex

• Nucleic acid

• Capture probe

• reporter probe

• Barcode

Which descriptions are appropriate in the "Purify and Immobilized" Step in the nCounter Analysis System workflow? Select all that apply?

• Excess probes are removed

• Load samples onto the cartridge

• count fluorescent barcodes bound to the cartridge

• Hybridized codeset and RNA complexes bind to the cartridge

Which of the following is NOT the application of nCounter technology?

• amplification of genes

• gene fusion analysis

• immune-oncology profiling

• gene expression analysis

What kinds of sample types can be used on nCounter Analysis system?

• cell lysate

• fresh frozen tissue

• whole blood

• All the above

Advantages of Nanostrings are ? Select all that apply.

• 800 hybridizations in a single tubes

• No amplification and reverse transcriptase

• Expression levels based on intensity of fluorescence

• simple workflow

The kind of magnifying tool that could be used to observe the surface of a red blood cell is a

• scanning electron microscope

• light microscope

• inverted microscope

• transmission electron microscope

The kind of magnifying tool that could be used to observe the internal parts of a cell is a

• scanning electron microscope

• light microscope

• inverted microscope

• transmission electron microscope

A microscope that bounces electrons off the surface of the specimen to produce a three-dimensional image.

• light microscope

• scanning electron microscope

• transmission electron microscope

• Fluorescence microscope

A scanning electron microscope uses _____ to produce an image.

• negatively charged particles

• positive charged particles

• neutral particles

• atoms

Which of the following samples are suitable for use of negative staining?

• Virus particles

• protein molecules

• bacteria flagella

• all the above