MI4.1.2d Green Fluorescent Protein

The unit of measure typically used to indicate the mass of a protein is

• kilodaltons

• daltons

• grams

• nanograms

• pounds

GFP has the ability to absorb ​ (a)   and emit ​ (b)   This process is called ​ (c)   .

• blue light
• green light.
• fluorescence
• bioluminescence
• red light

Tags

NGSS.HS-PS4-3

The part of the GFP protein responsible for light production is called the ________.

The gene coding for GFP can be altered to produce a variety of colors and light emission patterns.

• true

• false

Tags

NGSS.HS-LS3-2

Cells that are producing GFP will only fluoresce if exposed to

• UV light

• blue light

• water

• cold temperatures

• calcium salts

The gene for GFP can be inserted into a genome next to another gene in order to see when and where that gene is expressed. In this scenario, GFP is called a _______ gene.

In order to see GFP being expressed in specific locations in a cell or group of cells, you would need to use a

• microscope

• centrifuge

• incubator

• micropipette

• chromatography column

In column chromatography, lysed cells are added to the column. The cellular ​ (a)   move through a semi-solid (b)   that separates them. The matrix is held up in the column by the ​ (c)   , a porous disk at the bottom of the column. ​Liquid (d)   is run through to carry proteins down and out of the column.

• matrix
• frit
• buffer
• proteins
• GFP
• lipids
• water

A protein is said to ________ when is moves through and out of the chromatography column.

Which of the following is true of column chromatography in this lab? (Choose all that are true.)

• larger proteins will elute first

• smaller proteins will elute last

• smaller proteins will elute first

• larger proteins will elute last