12-02 Biotechnology

What do restriction enzymes do to DNA?

• glue together
• cut
• reconnect
• exchange

A _____ organism contains gene(s) from a different organism.

The process where a recombinant plasmid is inserted into a host cell is called ....

Which is not an example of a clone?

• Dolly the sheep

• Pest-resistant corn

• Identical twins

• A bacterial cell in a colony

Which would be a reason to NOT use genetically modified crops?

• increased crop yields

• reduced use of insecticide sprays

• feed more people faster

• increased pesticide resistance in pests

How might scientists check to make sure their GMO works? 

SELECT ALL THAT APPLY

• Add a fluorescent (glowing) gene (with the gene of interest) to see if the final product glows
• Add another gene (with the gene of interest), like a resistance to herbicide gene, to test for its addition in the final product.
• Add pests to the final product to see if they live or die.

Match the chromosomal mutation with its description

• Deletion

• When a segment of a chromosome breaks off, flips, and reattaches

• Translocation

• When a segment of a chromosome is added

• Insertion

• When a segment of a chromosome breaks and attaches to a non homologous chromosome

• Duplication

• When a segment of a chromosome is copied or doubles

• Inversion

• When a segment of a chromosome breaks off

What is one way CRISPR has already been used to change genes?

• Clone sheep
• Give people superpowers
• Cure animal diseases
• Military purposes

Using CRISPR, researchers have been able to remove genes from wheat to create a strain that is resistant to powdery mildew. This is an example of -

• selective breeding.

• a transgenic genetically modified organism.

• a non-transgenic genetically modified organism.

• an organically grown crop.

CRISPR can edit DNA. What organisms naturally has CRISPR?

• Viruses

• Humans

• Plants

• Bacteria