PTC Lab Procedure and Purpose

What is the purpose of x-tract buffer in PCR (Polymerase Chain Reaction) procedure?

• To amplify DNA fragments

• To increase the specificity of DNA amplification

• To facilitate denaturation of DNA

• Help extract DNA from cheek cells

What is the purpose of the Heat Block step at 10 minutes @ 95 degrees Celsius in PCR (Polymerase Chain Reaction) procedure?

• To cool down the reaction mixture before the next step

• break open cells - release DNA into solution

• To activate the DNA polymerase enzyme

• To denature the DNA into single strands

What is the function of Taq DNA Polymerase in the PCR (Polymerase Chain Reaction) procedure?

• Initiates the PCR reaction by denaturing the DNA template

• Adds primers to the DNA template for replication

• Facilitates the annealing of primers to the DNA template

• Catalyzes building complimentary strand during DNA synthesis (+ has a high heat capacity)

What is the function of PCR Buffer with Mg(^2) in the PCR (Polymerase Chain Reaction) procedure?

• Enhances enzyme activity and optimizes stability of primer template

• Maintains the optimal pH for enzyme activity

• Provides the necessary nucleotides for DNA synthesis

• Facilitates the annealing of primers to the DNA template

What is the function of dNTPs in the PCR (Polymerase Chain Reaction) procedure?

• Acts as a cofactor for DNA polymerase enzyme

• Facilitates the annealing of primers to the DNA template

• Provides the necessary nucleotides for DNA synthesis

• Provides the necessary energy for DNA amplification

What is the function of Gel loading dye in the PCR Master Mix?

• Provides additional charge to DNA molecules for better separation

• Stains the DNA to be seen for gel electrophoresis

• Adjusts the pH of the gel matrix

• Cuts the DNA to make bands

What is the function of PTC Primer Mix in PCR (Polymerase Chain Reaction) procedure?

• Increases the specificity of RNA amplification

• Facilitates denaturation of DNA strands

• Enhances the stability of the DNA template

• Contains complementary sequences to the target region for amplification

What is the function of denaturation in PCR (Polymerase Chain Reaction) procedure

• Breaks hydrogen bonds to form single-stranded genes

• Increases the stability of the DNA template

• Facilitates the annealing of primers to the DNA template

• Finishes the PCR reaction by bonding with the DNA template

What is the function of annealing in PCR (Polymerase Chain Reaction) procedure?

• Increases the temperature to denature the DNA template

• Promotes the elongation of the DNA strand

• Decreases temperature so primers attach to the template

• Facilitates the binding of DNA polymerase to the template strand

What is the function of extension in PCR (Polymerase Chain Reaction) procedure?

• Increases the temperature to build complementary strand

• Initiates the PCR reaction by denaturing the DNA template

• Facilitates the annealing of primers to the DNA template

• Increases the stability of the DNA template