MI - 1.4.2 - Vaccine Development

In the process above, restriction enzymes are used to cut the viral/donor DNA and the bacterial plasmid.  How many cuts are necessary?

• two cuts in the plasmid
• two cuts in the viral DNA
• one cut in the plasmid, two cuts in the viral DNA
• two cuts in the plasmid, one cut in the viral DNA

An injection delivered into the muscle layer

• subcutaneous
• intramuscular
• intradermal
• intravenal

An injection delivered just under the skin

• subcutaneous
• intramuscular
• intradermal
• intravenal

An injection delivered below the skin layer, but above the muscle layer

• subcutaneous
• intramuscular
• intradermal
• intravenal

What is herd immunity?

• Immunity acquired by a group of animals living together
• Immunity developed through natural selection in a herd of animals
• Immunity achieved when a high percentage of a population is vaccinated or immune to a contagious disease
• Immunity only observed in herds of domesticated animals

What is the significance of the recognition sequence for a restriction enzyme?

• It determines the enzyme's ability to cut DNA.
• It specifies the type of DNA base pairs it can recognize.
• It influences the enzyme's catalytic activity.
• It regulates the enzyme's expression in the cell.

Which of the following is an application of restriction enzymes in molecular biology?

• Sequencing DNA fragments
• Polymerase chain reaction (PCR)
• Transcription of mRNA from DNA templates
• Generating recombinant DNA molecules

Which enzyme was appropriate for us to use to make our recombinant DNA plasmid?

• HaeIII
• HindIII
• BamHI
• EcoRI
• Pst1

Why was it important to not disrupt the gene for antibacterial resistance?

• We needed this to grow it in an antibiotic medium to see if it took up the plasmid
• Since this is a bacteria, we needed to give the patient an antibiotic so the bacteria wouldn’t infect their cells
• The antibiotic gene works with the gene of origin to determine which one gets transcribed and translated
• It was not important as long as it didn’t get cut into more than two pieces

Tags

NGSS.HS-LS1-1

Which enzyme acts like glue to joint the complementary base pairs of the cut DNA on the plasmid?

• gyrase
• polymerase
• ligase
• helicase