MI - 1.4.2 - Vaccine Development

In the process above, restriction enzymes are used to cut the viral/donor DNA and the bacterial plasmid.  How many cuts are necessary?

• two cuts in the plasmid
• two cuts in the viral DNA
• one cut in the plasmid, two cuts in the viral DNA
• two cuts in the plasmid, one cut in the viral DNA

An injection delivered into the muscle layer

• subcutaneous
• intramuscular
• intradermal
• intravenal

An injection delivered just under the skin

• subcutaneous
• intramuscular
• intradermal
• intravenal

An injection delivered below the skin layer, but above the muscle layer

• subcutaneous
• intramuscular
• intradermal
• intravenal

What is herd immunity?

• Immunity acquired by a group of animals living together
• Immunity developed through natural selection in a herd of animals
• Immunity achieved when a high percentage of a population is vaccinated or immune to a contagious disease
• Immunity only observed in herds of domesticated animals

What is the significance of the recognition sequence for a restriction enzyme?

• It determines the enzyme's ability to cut DNA.
• It specifies the type of DNA base pairs it can recognize.
• It influences the enzyme's catalytic activity.
• It regulates the enzyme's expression in the cell.

Which of the following is an application of restriction enzymes in molecular biology?

• Sequencing DNA fragments
• Polymerase chain reaction (PCR)
• Transcription of mRNA from DNA templates
• Generating recombinant DNA molecules

Which enzyme was appropriate for us to use to make our recombinant DNA plasmid?

• HaeIII
• HindIII
• BamHI
• EcoRI
• Pst1

Why was it important to not disrupt the gene for antibacterial resistance?

• We needed this to grow it in an antibiotic medium to see if it took up the plasmid
• Since this is a bacteria, we needed to give the patient an antibiotic so the bacteria wouldn’t infect their cells
• The antibiotic gene works with the gene of origin to determine which one gets transcribed and translated
• It was not important as long as it didn’t get cut into more than two pieces

Which enzyme acts like glue to joint the complementary base pairs of the cut DNA on the plasmid?

• gyrase
• polymerase
• ligase
• helicase