Apps weeks 6 & 7 review

When DNA from two different organisms is combined it is called...

• Transgenic DNA

• Transformed DNA

• Recombinant DNA

• Spliced DNA

DNA from one organism can be inserted into another and used to produce a protein because...

• DNA is a triplet code

• The triplet code is universal

• The triplet code is degenerate

• The triplet code is non-overlapping

Which enzyme creates a single strand of DNA from mRNA?

• Reverse transcriptase

• Restriction endonuclease

• DNA ligase

• DNA polymerase

• RNA polymerase

Which enzyme cuts double stranded DNA at specific recognition sites?

• Reverse transcriptase

• Restriction endonuclease

• DNA ligase

• DNA polymerase

• RNA polymerase

Which enzyme joins a target gene of interest into the DNA of a vector?

• Reverse transcriptase

• Restriction endonuclease

• DNA ligase

• DNA polymerase

• RNA polymerase

Which enzyme produces double stranded DNA from single stranded DNA?

• Reverse transcriptase

• Restriction endonuclease

• DNA ligase

• DNA polymerase

• RNA polymerase

Which of the following sequences of DNA are palindromic?

• GAATTC

• GTATAC

• GCCGGC

• ACGCGT

Which of the following is NOT a way of obtaining a gene of interest?

• Using a gene machine

• Using reverse transcriptase

• Using restriction enzymes

• Using electrophoresis

ECOR I is a restriction enzyme that cutes DNA at the following base sequence: GAATTC. How many fragments of DNA would be produced if the restriction enzyme was used on this piece of DNA?

• 1

• 2

• 3

• 4

Why are restriction enzymes usually found in bacteria?

• To regulate gene expression in bacteria
• Defense mechanism against invading viruses and foreign DNA
• Used for energy production in bacteria
• To help bacteria communicate with each other