BMD - Primers & Probes

Which technique uses primers?

• Southern blot

• ELISA

• PCR

• Microarray

A primer is a short _____ sequence that binds to its _____ sequence in the target.

• DNA; complementary

• DNA; exact

• protein; DNA

• RNA; exact

What is the sequence of the primer that binds to this target DNA?

5'-GTACTAGGATTAATGCGTTT-3'

• 5'-GTACTAGGATTAATGCGTTT-3'

• 3'-GTACTAGGATTAATGCGTTT-5'

• 5'-AAACGCATTAATCCTAGTAC-3'

• 3'-AAACGCATTAATCCTAGTAC-5'

Which primer characteristic DOES NOT affect its SPECIFICITY?

• Length

• GC content

• Uniqueness

• Melting temperature

What is the melting temperature (Tm) for this primer?

5'-TCA GTG ATG GTG ATG ATG GCG-3'

• 56'C

• 60'C

• 54'C

• 64'C

What is the annealing temperature for the following primer pair?

Forward primer Tm = 60'C

Reverse primer Tm = 58'C

• 56'C

• 58'C

• 60'C

• 62'C

Which online tool should be used to check for primer specificity?

• PubMed

• blast

• NCBI

• UniProt

A probe is different from a primer because

• probe can have modification at its 3' end.

• probe must always be longer than a primer.

• probe is usually RNA while primer is DNA.

• probe binds outside the gene of interest.

Which one of the following is NOT the result of using a short primer?

• Low Tm

• Low specificity

• Low sensitivity

• Low stability

A probe can be used in

• real-time PCR.

• gel electrophoresis.

• conventional PCR.

• sequencing.