MI 2.1.2 Experiment Review

What is the objective of the Edvo-Kit #330 experiment?

• To understand the structure of DNA

• To gain hands-on experience of the principles and practice of Polymerase Chain Reaction (PCR)

• To learn about the storage instructions for laboratory equipment

• To study the effects of temperature on enzyme activity

How are the PCR products analyzed in the Edvo-Kit #330 experiment?

• Through spectrophotometry

• By agarose gel electrophoresis

• Using a microscope

• By measuring the pH level

Who was awarded the Nobel Prize in Chemistry in 1993 for the development of PCR?

• Kary Mullis

• Thomas Brock

• Alec Jeffreys

• Frederick Sanger

What is the optimal temperature for the "extension" step in PCR?

• 45°C - 65°C

• 94°C - 96°C

• 72°C

• 37°C

During which step of PCR does the temperature rise to denature the DNA strands?

• Annealing

• Extension

• Denaturation

• Initial heating

How many cycles of PCR are typically required to produce enough DNA for analysis?

• 5 to 10 cycles

• 20 to 25 cycles

• 30 to 35 cycles

• 40 to 45 cycles

What is the first step in the PCR cycle depicted in the diagram?

• Anneal 2 primers at 40°C - 65°C

• Denature at 94°C

• Extension at 72°C

• Separation of two DNA strands

During which step of the PCR cycle are the primers annealed?

• Denature at 94°C

• Anneal 2 primers at 40°C - 65°C

• Extension at 72°C

• Separation of two DNA strands

At what temperature does the extension step occur in the PCR cycle shown in the diagram?

• 94°C

• 40°C - 65°C

• 72°C

• 55°C

How many DNA strands are produced from one target sequence after three cycles of PCR as shown in the diagram?

• 2

• 4

• 8

• 16