Chromatography Quiz

What does Hydrophobic Interaction Chromatography (HIC) exploit to separate molecules?

• Molecular charge

• Hydrophobicity differences

• Molecular size

• Affinity for nickel ions

What is the basis for separation in Ion Exchange Chromatography?

• Hydrophobicity differences

• Molecular size

• Charge properties of molecules

• Affinity for histidine tags

Size Exclusion Chromatography is also known as:

• Affinity chromatography

• Gel filtration chromatography

• Ion exchange chromatography

• Hydrophobic interaction chromatography

What is the principle behind the separation of molecules in Size Exclusion Chromatography (SEC)?

• Charge properties

• Hydrophobicity

• Molecular size

• Affinity for nickel ions

What type of molecules does Ni-NTA Agarose Chromatography specifically purify?

• Molecules with a positive charge

• Proteins with a polyhistidine (His-tag) sequence

• Molecules based on their size

• Hydrophobic molecules

Explain the principle behind Hydrophobic Interaction Chromatography.

• It separates molecules based on their size.

• It separates molecules based on their charge.

• It separates molecules based on their hydrophobic interactions.

• It separates molecules based on their ability to bind to metal ions.

How does Ion Exchange Chromatography separate molecules?

• By their hydrophobic interactions.

• Based on their charge properties.

• By their size.

• By their specific binding to metal ions.

What is the mechanism of Size Exclusion Chromatography?

• It separates molecules by their ability to bind to metal ions.

• It separates molecules by their charge.

• It separates molecules by their hydrophobic interactions.

• It separates molecules based on their size.

What is the purpose of Ni-NTA Agarose Chromatography?

• To separate molecules based on their hydrophobic interactions.

• To separate molecules based on their charge.

• To separate molecules based on their size.

• To purify His-tagged proteins by specific binding to metal ions.

Which type of chromatography would be most suitable for separating a mixture of positively charged and negatively charged molecules?

• Hydrophobic Interaction Chromatography

• Ion Exchange Chromatography

• Size Exclusion Chromatography

• Ni-NTA Agarose Chromatography