DNA Replication

This structure is an Okazaki fragment.

• A

• D

• F

• G

H is the

• leading strand

• lagging strand

• parent strand

• none of these

Which enzyme proofreads and removes RNA Primase

• Ligase

• Polymerase 1

• Polymerase 3

• Helicase

Which enzyme is responsible for adding nucleotides to the free three prime end

• Helicase

• DNA Gyrase (Topoisomerase)

• DNA Polymerase 3

• DNA Polymerase 1

Okazaki fragments are found on the _______________ strand

• Leading

• Lagging

This enzyme synthesizes short RNA sequences to guide DNA Polymerase

• Primers

• DNA Primase

• DNA Polymerase

• Topoisomerase

In the Meselson-Stahl experiment, E.coli was transfered from ¹⁵N medium to ¹⁴N medium. Centrifugation of DNA after 20 minutes in ¹⁴N produced ____ and disproved the ____ model.

• 1 intermediate band, conservative

• 1 intermediate band, dispersive

• 1 heavy band and 1 light band, semi-conservative

• 1 light and 1 intermediate band, dispersive

What does PCR stand for?

• Polymerase Cytoplasmic Reaction

• Polymerase Chromosomal Reaction

• Polymerase Chain Reaction

• Probably Cannot React

Answer explanation

At what temperature does the denature step of PCR occur?

• 94-96° C

• 50-65° C

• 72° C

• Any temperature

Answer explanation

It has to be HOT, HOT, HOT!

Tags

NGSS.HS-PS1-5

During PCR, the temperature is increased very high. What is the purpose of this?

• DNA replication

• Strand Separation

• Binding Primers

• Denaturing the enzymes involved

Answer explanation

High temperatures will break the hydrogen bonds between the DNA strands.