PHYSCI125 Midterm Review

Given this template strand sequence, what is the sequence of the RNA transcript?

Template strand: 5’ - ACTGAGAACC - 3’

• 5’ - ACTGAGAACC - 3’

• 5’ - GGUUCUCAGU - 3’

• 5’ - UGACUCUUGG - 3’

• 5’ - GGTTCTCAGT - 3’

Which of the following is usually NOT found in a mature mRNA transcript? (Select all that apply)

• 5’ UTR

• Introns

• 3’ UTR

• Poly-A tail

• Promoter

Where are the 5’ss and 3’ss “critical nucleotide” signals found in the following gene sequence?

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Categorize each of these characteristics into exonic (protein-coding) traits vs. intronic (non-coding) traits:

CpG islands early in the sequence, Enrichment of preferred codons, Lower GC content, Presence of AUG early in the sequence, Longer sequence length, Sequence similarity to known ESTs, Highly repetitive

• Exons: CpG islands early in the sequence, Enrichment of preferred codons, Presence of AUG early in the sequence, Sequence similarity to known ESTs

• Introns: Lower GC content, Longer sequence length, Highly repetitive

• Exons: Lower GC content, Longer sequence length, Highly repetitive

• Introns: CpG islands early in the sequence, Enrichment of preferred codons, Presence of AUG early in the sequence, Sequence similarity to known ESTs

What is the GC content of your sequence?

• 3/9

• 4/9

• 5/9

• 6/9

What kind of alternative splicing is present at exon 2 for this sense gene?

• Exon skipping

• Intron retention

• Alternative 5'ss

• Alternative 3'ss

What kind of alternative splicing is present at exon 2 for this antisense gene?

• Exon skipping

• Intron retention

• Alternative 5'ss

• Alternative 3'ss

Where do miRNAs most often bind?

• 5’ cap of a mature mRNA transcript

• 3’ end of a peptide chain

• Intronic regions on the DNA

• 3' UTRs of RNA transcripts

Which of the following is NOT an effect of miRNA binding?

• RNA transcript destabilization

• RNA transcript replication

• RNA transcript degradation

• Prevention of translation initiation

Which of the following are true about the seed region?

• It is a region on the miRNA between 2-8 nt in length

• It is often conserved across multiple species

• The best target regions on RNAs will have perfect sequence complementarity with the seed region

• All of the above